Publications from the Slepak Lab
Selected Meeting Presentations | Invited Seminars
Narayanan V, Sandiford SL, Wang Q, Keren-Raifman T, Levay K, Slepak VZ. Intramolecular Interaction between the DEP Domain of RGS7 and the Gbeta(5) Subunit. Biochemistry. 2007 May 19
Rosenzweig DH, Nair KS, Wei J, Wang Q, Garwin G, Saari JC, Chen CK, Smrcka AV, Swaroop A, Lem J, Hurley JB, Slepak VZ. Subunit dissociation and diffusion determine the subcellular localization of rod and cone transducins. J Neurosci. 2007 May 16;27(20):5484-94.
Nair KS, Hanson SM, Mendez A, Gurevich EV, Kennedy MJ, Shestopalov VI, Vishnivetskiy SA, Chen J, Hurley JB, Gurevich VV, Slepak VZ. "Light-dependent redistribution of arrestin in vertebrate rods is an energy-independent process governed by protein-protein interactions," Neuron (2005) 46(4):555-67.
Nair KS, Mendez A, Blumer JB, Rosenzweig DH, Slepak VZ. "The presence of a Leu-Gly-Asn repeat-enriched protein (LGN), a putative binding partner of transducin, in ROD photoreceptors," Invest Ophthalmol Vis Sci. (2005) 46(1):383-9.
Nair KS, Hanson SM, Kennedy MJ, Hurley JB, Gurevich VV, Slepak VZ. "Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors", J Biol Chem. (2004) 279(39):41240-8.
Gutierrez-Ford C, Levay K, Gomes AV, Perera EM, Som T, Kim YM, Benovic JL, Berkovitz GD, Slepak VZ. "Characterization of tescalcin, a novel EF-hand protein with a single Ca2+-binding site: metal-binding properties, localization in tissues and cells, and effect on calcineurin," Biochemistry (2003) 42(49):14553-65.
Balasubramanian N, Slepak VZ. "Light-mediated activation of Rac-1 in photoreceptor outer segments," Curr Biol. (2003) 13(15):1306-10.
Rishal I, Keren-Raifman T, Yakubovich D, Ivanina T, Dessauer CW, Slepak VZ, Dascal N. “Na+ promotes the dissociation between Galpha- GDP and Gbeta-gamma, activating G-protein-gated K+ channels" J Biol Chem (2003) 278(6):3840-3845
Witherow DS, Tovey SC, Wang Q, Willars GB, Slepak VZ. “Gbeta 5-RGS7 inhibits Galpha q-mediated signaling via a direct protein-protein interaction” J Biol Chem. (2003) 278(23):21307-13.
Witherow DS & Slepak VZ “A Novel Kind of G Protein Heterodimer: The Gbeta5-RGS Complex.” Receptors Channels. (2003) 9(3):205-12. (review)
Hausmann ON, Hu WH, Keren-Raifman T, Witherow DS, Wang Q, Levay K, Frydel B, Slepak VZ1, Bethea JR1 "Spinal cord injury induces expression of RGS7 in microglia/macrophages in rats." European Journal of Neuroscience (2002),15(4):602-12.
1- corresponding author.
Nair KS, Balasubramanian N & Slepak VZ "Signal-Dependent Translocation of Transducin, RGS9-1-Gb5L Complex and Arrestin to Detergent-Resistant Membrane Rafts in Photoreceptors". Current Biology (2002), 12, 421-425.
Balasubramanian N, Levay K, Keren-Raifman T, Faurobert E, & Slepak VZ "Phosphorylation of Regulator of G protein Signaling RGS9-1 by Protein Kinase A is a Potential Mechanism of Light- and Ca2+-mediated Regulation of G protein Function in Photoreceptors", Biochemistry (2001), 40, 12619-27.
Keren-Raifman T, Bera AK, Zveig D, Peleg S, Witherow DS, Slepak VZ, Dascal N. "Expression levels of RGS7 and RGS4 proteins determine the mode of regulation of the G protein-activated K(+) channel and control regulation of RGS7 by G beta 5". FEBS Lett. 2001 Mar 9;492(1-2):20-8.
Witherow, D. S., Wang, Q., Levay, K, Cabrera. J. L., Chen, J., Willars, G. and Slepak, V. Z. "Complexes of the G protein subunit Gb5 with regulators of G protein signaling RGS7 and RGS9: characterization in native tissues and in transfected cells". J. Biol. Chem. (2000), 275, 24872-24880.
Satpaev, D. K. and Slepak, V.Z. "Analysis of protein-protein interactions in the phototrans-duction cascade using surface plasmon resonance". Methods in Enzymology, (2000), 316, 20-40.
Slepak, V. Z. "Application of Surface Plasmon Resonance for Analysis of Protein-Protein Interactions in the G Protein-Mediated Signal Transduction Pathway". Review. Journal of Molecular Recognition, (2000) 13(1) 20-26
Levay, K. Cabrera, J. L. Satpaev, D. K. and Slepak, V.Z. "Gb5 prevents the RGS7-Gao interaction through binding to a distinct Gg-like domain found in RGS7 and other RGS proteins" Proc. Natl. Acad.Sci, (1999) 96, 2503-2507.
Cabrera, J. L. de Freitas, F., Satpaev D. K., and Slepak, V.Z. "Identification of the Gb5-RGS7 Protein Complex in the Retina." Biochim. Biophis. Res. Commun. (1998), 249, 898-902.
Levay, K. Satpaev, D.K., Pronin, A.N., Benovic, J.L. and Slepak, V.Z. "Localization of the sites for Ca2+-binding proteins on G protein-coupled receptor kinases", Biochemistry, (1998), 37, 13650-59.
Craft, C. M., Xu, J. Slepak, V. Z., Zhan, X., Zhu, X., Brown, B. and Lolley, R. N. "PhLPs and PhLOPs in the Phosducin Family of Gbg Binding Proteins" Biochemistry, (1998) 37, 15758-15772.
Satpaev, D. K., Chen, C.-K., Scotti, A., Simon, M. I., Hurley, J. B. and Slepak, V.Z. "Autophosphorylation and ADP binding regulate the Ca2+-dependent interaction of recoverin with rhodopsin kinase", Biochemistry, (1998) 37, 10256-10262.
Pronin, A. N., Satpaev, D. K. Slepak, V. Z. and Benovic, J. L. "Regulation of G Protein-Coupled Receptor Kinases by Calmodulin and Localization of the Calmodulin Binding Domain" J. Biol. Chem. (1997), 272, 18273-79
Yu, B., Slepak, V. Z., and Simon, M. I. "Characterization of a Goalpha Mutant that binds Xantine nucleotides", J. Biol. Chem. (1997), 272, 18015-18019
Watson, J. A., Aragay, A. M., Slepak, V. Z., and Simon, M. I. "A novel form of the G protein b subunit Gb5 specifically expressed in vertebrate retina", J. Biol. Chem., (1996), 271, 28154-60
Doupnik, C. A., Dessauer, C. W., Slepak, V. Z., Gilman, A. G., Davidson, N., and Lester, H. A. "Time resolved Kinetics of Direct Gb1g2 Interactions with the Carboxyl Terminus of Kir3.4 Inward Rectifier K Channel Subunits", Neuropharmacology, (1996) 923-931.(1996-2002
Selected meeting presentations: Listed by year, with only the presenting author indicated. |
Ford, C., “Tescalcin: a novel ca2+-binding protein with single EF-hand” Gordon Research Conference on Second messengers and protein phosphorylation. Meriden NH. |
| Nair, S. “Mechanism of localization of arrestin to lipid rafts” Gordon Research Conference on Second messengers and protein phosphorylation. Meriden NH. |
| Nair, S . “Translocation of transducin, RGS9 and arrestin to lipid rafts in photoreceptors: mechanisms and effects” ARVO annual Meeting. Ft. Lauderdale, FL. |
| Slepak, V. “Localization of the G protein, RGS and arrestin in lipid rafts” FASEB Summer Research Conference, Biology and Chemistry of Vision. Tucson, AZ. |
| Slepak, V. Localization of the G protein, RGS and arrestin in lipid rafts” Gordon Research Conference, Molecular Pharmacology, Ventura, CA. |
Slepak, V. Z., "Signal-induced translocation of G protein, Arresting and RGS9-Gbeta5L complex to lipid rafts in photoreceptors" (2002) G protein Workshop, Mount Sinai Hospital, New York, NY. |
| Nair, S.. "Signal-Dependent Translocation of Transducin, RGS9-1-Gb5L Complex and Arrestin to Detergent-Resistant Membrane Rafts in Photoreceptors" Gordon Research Conference on Second Messengers & Protein Phosphorylation, Meriden, NH. |
Balasubramanian, N. "Phosphorylation of Regulator of G protein Signaling RGS9-1 in
Photoreceptors", ASBMB-ASPET Annual Meeting, New Orleans, LA. |
| Witherow, D. S. "Gb5-RGS complex: a novel kind of G protein heterodimer" ASBMB-ASPET Annual Meeting, New Orleans, LA. |
| Slepak, V. Z. et al, "Assembly and function of Gb5-RGS complexes" (2001) Gordon Research Conference on Molecular Pharmacology, Ventura, CA. |
| Witherow, D. S., Wang, Q., et al "Assembly and function of Gb5-RGS complexes" (2001) ASBMB-ASPET Annual Meeting, Orlando, FL |
| Slepak, V. Z. et al "Assembly and function of Gb5-RGS complexes"; "Phosphorylation of Regulator of G protein Signaling RGS9-1 by Protein Kinase A is a Potential Mechanism of Light- and Ca2+-mediated Regulation of G protein Function in Photoreceptors" (2001) 11th International Conference On Second Messengers & Phosphoproteins, Melbourne, Australia |
| Balasubramanian, N., et al. "Phosphorylation of Regulator of G protein Signaling RGS9-1 by Protein Kinase A is a Potential Mechanism of Light-and Ca2+-mediated Regulation of G protein Function in Photoreceptors", (2001) 11th International Conference On Second Messengers & Phosphoproteins, Melbourne, Australia (Oral presentation) |
| 2000 |
Slepak VZ et. al, "Analysis of Gb5-RGS7 complexes in vivo and in vitro" Keystone Symposia, Keystone, CO |
Witherow DS et. al, "Gb5-RGS7 complexes in vivo and in vitro" Keystone Symposia, NM |
| Witherow DS et. al, "Complexes of Gb5 with regulators RGS7 proteins in vivo and in vitro" ASBMB-ASPET Annual Meeting, Boston, MA |
Levay K et. al, "Gb5 Subunit binds to RGS proteins with distinct Gg -Like domain and inhibits RGS-Ga interaction" Signal Transduction and Therapeutic Strategies, Miami Nature Biotechnology Winter Symposium
Satpaev DK et. al, The Study Of Interaction Between G-Protein Coupled Receptor Kinases and Ca2+-Binding Proteins., Miami Nature Biotechnology Winter Symposium
Witherow DS and Slepak VZ The RGS7-Gb5 complex in rat brain. Signal Transduction and Therapeutic Strategies, Miami Nature Biotechnology Winter Symposium
Witherow DS et al Complexes of the G protein beta 5 subunit and RGS proteins in the retina, FASEB Summer Research Conference, Copper Mountain, CO
.Levay K et. al, "Interaction of GRKs with Calcium-Binding Proteins". Molecular Pharmacology Gordon Research Conference, Ventura CA.
Satpaev DK et. al, Interaction Between G-Protein Coupled Receptor Kinases and Ca2+-Binding Proteins., FASEB Summer Research Conference, Copper Mountain, CO.
Slepak VZ et. al, "The interaction of Gb5 with RGS proteins: a mechanism to regulate the Regulator" Molecular Pharmacology Gordon Conference, Ventura CA. (Oral Presentation)
Slepak VZ "Gb5-RGS interaction". PhRMA Foundation Annual Meeting, New York, NY.
Slepak VZ et. al, "Gb5 is a Gb subunit that does not have a Gg and interacts with RGS proteins" Gordon Research Conference on Second Messengers & Protein Phosphorylation, Meriden, NH (Oral presentation)
Slepak VZ "Approaches to studying the G Protein Cascade by BIA on lipid surfaces" 9th annual BIAsymposium, San Francisco, CA 1999 (Invited speaker presentation)
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Cabrera JL et. al, "Forms of G protein subunit Gb5 expressed in retina". ARVO Annual Meeting, Fort Lauderdale, FL
Satpaev DK et. al, "Interaction of Rhodopsin Kinase with Recoverin and Calmodulin". ARVO Annual Meeting, Fort Lauderdale, FL
Slepak VZ "Kinetic Analysis of the G Protein Cascade." 8th annual BIAsymposium, Edinburgh, Scotland, UK (Oral presentation)
Slepak VZ "Purification of a 55 kDa Gb5-binding protein". PhRMA Foundation Annual Meeting, New York, NY
Slepak VZ et. al, "G protein beta subunit, Gb5, interacts with RGS7". 10th International Conference on Second Messengers & Phosphoproteins, Jerusalem, Israel (Oral presentation) Slepak VZ et. al, "Interaction of G protein-coupled Receptor Kinases with Calcium-Binding Proteins". 10th International Conference on Second Messengers & Phosphoproteins, Jerusalem, Israel, (Oral presentation) Cabrera JL et. al, "Forms of G protein subunit Gb5 expressed in retina". ASBMB annual Meeting, Washington DCSatpaev DK et. al, "Interaction of Rhodopsin Kinase with Recoverin and Calmodulin" ASBMB annual Meeting, Washington DC
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Slepak VZ et. al, "Rhodopsin Kinase - Recoverin Interaction on BIAcore". ARVO Annual Meeting, Fort Lauderdale, FL (Oral presentation)
Slepak VZ "Interaction of Ca2+-binding proteins with GRKs" Gordon Research Conference on Second Messengers & Protein Phosphorylation. (Invited speaker presentation)
Slepak VZ "Temporal resolution of G protein-mediated signaling" 7th annual BIAsymposium, Marina del Ray, CA. (Invited speaker presentation)
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Slepak VZ "Analysis of the G protein cascade by BIA." 6th annual BIAsymposium, Washington, DC (Invited speaker presentation)
Cabrera JL et. al, "Forms Of The Novel G Protein b Subunit, Gb5, Expressed In Retina." FASEB Keystone Symposia, Keystone, CO.
Slepak VZ "Analysis of protein-protein interactions in the G protein cascade using BIAcore biosensor." 5th annual BIAsymposium, San Diego, CA (Invited speaker presentation) |
Invited Seminars (Vlad Slepak) |
"Gbeta5-RGS complex: a novel kind of G protein heterodimer" (2002)
Columbia University, New York, NY."Novel mechanisms in G protein Signaling" (2001)
The Centre for Molecular Biology and Medicine Melbourne, Australia"Identification and study on a novel type of G protein heterodimer, Gb5-RGS complex" (2001)
Virginia Commonwealth University Richmond, VA"Novel mechanisms in G protein Signaling" (2000)
Georgia Institute of Technology, Atlanta, GA"Identification and study on a novel type of G protein heterodimer, Gb5-RGS complex" (2000)
Oregon Health Sciences University, Portland, OR "Gb5-RGS interaction: a story told by an orphan Gb subunit" (1999)
National Institutes of Health, Bethesda, MD.
"Gb5 is a Gb subunit that does not have a Gg and interacts with RGS proteins" (1999)
SUNY at Stony Brook, Stony Brook, NY. "Interaction of RGS proteins with G protein beta subunit Gbeta5" (1998)
Free University of Berlin, Berlin, Germany. "Interaction of G protein-coupled receptor kinases with Ca2+-binding proteins" (1998)
Centro de Biologia Molecular, University of Madrid, Madrid, Spain. "Regulation of G protein-coupled receptor kinases by Ca2+-binding proteins" (1998)
National Institutes of Health, Bethesda, MD. "Temporal resolution of protein-protein interactions in rhodopsin cascade" (1997)
University of Southern California, Los Angeles, CA. "Regulation of G protein-coupled receptor kinases by Ca2+-binding proteins" (1997)
Harvard University, Boston MA. "Kinetic analysis of protein-protein interactions in the G protein cascade using SPR" (1996)
Bristol Meyers Squibb, Inc., Wallingford, CT. "Analysis of the G protein cascade using Surface Plasmon Resonance" (1996)
Yale University, New Haven, CT. "Novel G protein b Subunit, Gb5" (1996)
Cadus Inc, Terrytown, NY. "Using biosensor technology for investigation of signal transduction pathways" (1996)
New York University, New York, NY. |
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