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------------------------------------------------------------------------------------------------- Curriculum Vitae B.A.,
Biology, Rice University, Houston 1962 ------------------------------------------------------------------------------------------------- Research Interests Many
aspects of the process of cellular transformation that leads to
cancer in vivo can be studied in cultured cells. My interests have
focused on the dramatic changes in shape and behavior that occur
in normal cultured cells when they are treated with the transforming
growth factors, TGF-" and TGF-B. NRK cells growing normally
will spread out and adhere tightly to the culture dish. Internally,
the cellular actin cytoskeleton is organized into an extensive system
of filament bundles that attach to focal adhesions and stabilize
cell shape. Within a few hours of the application of transforming
growth factors, the actin filament bundles are disrupted and the
cells adopt a spherical shape that is weakly adherent to the dish.
This radical structural change in the cell shape and actin organization
is correlated with the down-regulation of isoforms of tropomyosin,
a protein that binds to and stabilizes actin filaments. I am particularly
interested in the mechanisms of transformation that induce the change
in expression level of tropomyosin and how its loss contributes
to the cytoskeletal breakdown and cell shape remodeling, using methods
of cell culture, cell microinjection, and fluorescence imaging of
live cells. ------------------------------------------------------------------------------------------------- Selected Publications C. L. Leonardi, R. H. Warren, and R. W. Rubin. Lack of tropomyosin correlates with the absence of stress fibers in transformed rat kidney cells. Biochim. Biophys. Acta 720:154-162 (1982). R. H. Warren, E. Gordon and R. Azarnia. Tropomyosin in peripheral ruffles of cultured rat kidney cells. Eur. J. Cell Biol. 38:245-253 (1985). R. H. Warren. TGF-a-induced breakdown of stress fibers and degradation of tropomyosin in NRK cells is blocked by a proteasome inhibitor. Experimental Cell Res. 236:294-303 (1997).
View published research articles by Dr. Warren in the National Library of Medicine
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